When a sample solution containing various
molecules slowly flows through the gel column, each molecule in the
column simultaneously with two different motion: vertical downward
movement and non-directed diffusion movement. Macromolecules
due to a larger diameter, not easy to enter pores of the gel particles,
but only the distribution between the particles, the moving down when
eluted faster. After the addition to
the proliferation of small molecules in the gel particle gap, you can
also enter the pores of the gel particles that enter the gel phase in
the process of moving down in a gel diffusion from the particle gap then
enter another gel particles, so continue to enter and spread down the
speed of small molecules behind the macromolecules so large molecules in
the sample elutes from the column first, slower middle molecules, molecular sieve manufacturers smallest final outflows, a phenomenon called zeolite adsorbent zeolite desiccant effect.
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