Molecular sieve chromatography

molecular sieve chromatography, also known as gel or gel filtration chromatography. Gel filtration is the use of a certain pore size range of porous gel as the stationary phase, the components of the mixture according to molecular size separating chromatographic technique. A substance having a 13x molecular sieves action are many, such as pumice, agar, agarose, polyvinyl alcohol, polyacrylamide, dextran gels and the like. Sephadex most widely trade name sephadex many models, from G10 to G200, its main applications are: ① fractionation various antigens and antibodies; ② remove complexes of small molecules. Such as desalination, fluorescein and free radioisotopes as well as protein hydrolysis fragments; ④ Determination of the molecular weight; ③ Analysis of immune complexes in serum.